Unity 2020.3.33f1 crashes when run the active scene first time

Yesterday I got this error: (before it worked like a charm)

I have a big scene in unity. When I load up the project, the active scene will be this large scene. I hit PLAY button -> the memory increases to 20 GB and crashes.

But the weird thing is:

When I change to SCENE2 hit play->work after that I change SCENE1 hit play->work (with 3 GB usage, and 3-4 seconds load)

So basically Unity 2020.3.33f1 crashes when first time load the SCENE1 and hit PLAY