Can I use multi-layered textures on GUI?

So I've been making an FPS game and I've gotten to the point of making the GUI, I wanted to do a Call of Duty styled bullet counter where you see the bullets and as you shoot they vanish. I know I can get this effect by having multiple images of the GUI with bullets removed, but can I use a multilayered image made in a program such as GIMP to get the same effect?