Google Mobile Ads Interstitial ads is not showing in unity

hi,am downloaded the Google Mobile Ads plugin from their website ,I follow the steps what they have,Banner Ads is Showing but Interstitial Ads is not,Here is my code..

 using UnityEngine;
 using System.Collections;
 using System.Collections.Generic;
 using GoogleMobileAds.Api;
 using GoogleMobileAds;
 
 public class AdsShowTest : MonoBehaviour {
 
 
     private BannerView bannerView;
     private InterstitialAd interstitial;
 
 
 
     void Start () 
     {
         DontDestroyOnLoad(this);
         RequestBanner ();
         RequestInterstitial ();
 
 
     }
     public void RequestBanner()
     {
         #if UNITY_ANDROID
         string adUnitId = "ca-app-pub-3940256099942544/6300978111";
         #elif UNITY_IPHONE
         string adUnitId = "INSERT_IOS_BANNER_AD_UNIT_ID_HERE";
         #else
         string adUnitId = "unexpected_platform";
         #endif
 
         // Create a 320x50 banner at the top of the screen.
         BannerView bannerView = new BannerView(adUnitId, AdSize.Banner, AdPosition.Top);
         // Create an empty ad request.
         AdRequest request = new AdRequest.Builder().Build();
         // Load the banner with the request.
         bannerView.LoadAd(request);
         //bannerView.Show();
     }
 
     private void RequestInterstitial()
     {
         #if UNITY_ANDROID
         string adUnitId = "ca-app-pub-3940256099942544/1033173712";
         #elif UNITY_IPHONE
         string adUnitId = "INSERT_IOS_INTERSTITIAL_AD_UNIT_ID_HERE";
         #else
         string adUnitId = "unexpected_platform";
         #endif
         
         // Initialize an InterstitialAd.
         InterstitialAd interstitial = new InterstitialAd(adUnitId);
         // Create an empty ad request.
         AdRequest request = new AdRequest.Builder().Build();
         // Load the interstitial with the request.
         interstitial.LoadAd(request);
         print("Ad is Loaded");
 
         if (interstitial.IsLoaded()) 
         {
             interstitial.Show();
             print("Ad is Showing");
         }
     }
     
 
 
     void Update ()
     {
 
 
 
         if(Input.GetKeyDown(KeyCode.Escape))
            Application.Quit();
 
     }
 
 }

i Modify the Ad Manifest also, Please tell me what am doing wrong.Thanks